Composite

Part:BBa_K4803109:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


CD4signal-FRB-CD28TMD-TEV CS-tTA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 303
    Illegal BamHI site found at 1139
    Illegal XhoI site found at 88
    Illegal XhoI site found at 169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part.

Source

This biobrick is MESA Part created by combining Part:BBa_K4803005(CD4 signal), Part:BBa_K4803003(FRB), Part:BBa_K4803006(CD28 Transmembrane domain), Part:BBa_K1362453(TEV cleavage site), Part:BBa_K2696013(tTA).